Primer
Part:BBa_K2945012:Design
Designed by: Cian Colgan Group: iGEM19_UChicago (2019-10-15)
Cut 4 Primer: Primer opposite the Prefix Binding Site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 11
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 11
Illegal NotI site found at 3 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 11
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 11
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 11
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Primer is Compatible for Linearization by PCR with the Cut 3 Primer that is opposite the VF2 Binding Site (BBa_K2945011)
Source
pSB1C3