Primer

Part:BBa_K2945012:Design

Designed by: Cian Colgan   Group: iGEM19_UChicago   (2019-10-15)


Cut 4 Primer: Primer opposite the Prefix Binding Site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 11
    Illegal NotI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 11
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Primer is Compatible for Linearization by PCR with the Cut 3 Primer that is opposite the VF2 Binding Site (BBa_K2945011)


Source

pSB1C3

References